Introduction of Culex Toxicity into Bacillus thuringiensis Cry4Ba by Protein Engineering
نویسندگان
چکیده
منابع مشابه
Cyt1A from Bacillus thuringiensis restores toxicity of Bacillus sphaericus against resistant Culex quinquefasciatus (Diptera: Culicidae).
The 2362 strain of Bacillus sphaericus, which produces a binary toxin highly active against Culex mosquitoes, has been developed recently as a commercial larvicide. It is being used currently in operational mosquito control programs in several countries including Brazil, France, India, and the United States. Laboratory studies have shown that mosquitoes can develop resistance to B. sphaericus, ...
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Bacillus thuringiensis (Bt) is a valuable environmentfriendly biopesticide, which occupies 90% of the world biopesticide market. Its insecticidal properties are attributed to the presence of δ-endotoxins which are synthesized during the sporulation phase of the bacterium. δ-endotoxin or crystal toxin is a multidomain protein molecule comprising of three distinct domains. Domain I is made of sev...
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متن کاملFunctional characterizations of residues Arg-158 and Tyr-170 of the mosquito-larvicidal Bacillus thuringiensis Cry4Ba
The insecticidal activity of Bacillus thuringiensis (Bt) Cry toxins involves toxin stabilization, oligomerization, passage across the peritrophic membrane (PM), binding to midgut receptors and pore-formation. The residues Arg-158 and Tyr-170 have been shown to be crucial for the toxicity of Bt Cry4Ba. We characterized the biological function of these residues. In mosquito larvae, the mutants R1...
متن کاملProduction of chymotrypsin-resistant Bacillus thuringiensis Cry2Aa1 delta-endotoxin by protein engineering.
Cleavage of the Cry2Aa1 protoxin (molecular mass, 63 kDa) from Bacillus thuringiensis by midgut juice of gypsy moth (Lymantria dispar) larvae resulted in two major protein fragments: a 58-kDa fragment which was highly toxic to the insect and a 49-kDa fragment which was not toxic. In the midgut juice, the protoxin was processed into a 58-kDa toxin within 1 min, but after digestion for 1 h, the 5...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 2003
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.69.9.5343-5353.2003